› 4ph8

X-ray diffraction
1.55Å resolution

Crystal structure of AggA, the major subunit of aggregative adherence fimbriae type I (AAF/I) from the Escherichia coli O4H104

Released:
DOI: 10.2210/pdb4ph8/pdb
PDB entry 4ph8 coloured by chain, front view.
PDB entry 4ph8 coloured by chain, side view.
PDB entry 4ph8 coloured by chain, top view.
Source organism: Escherichia coli O104:H4 str. C227-11
Primary publication:
Structural insight into host recognition by aggregative adherence fimbriae of enteroaggregative Escherichia coli.
Berry AA, Yang Y, Pakharukova N, Garnett JA, Lee WC, Cota E, Marchant J, Roy S, Tuittila M, Liu B, Inman KG, Ruiz-Perez F, Mandomando I, Nataro JP, Zavialov AV, Matthews S
PLoS Pathog 10 e1004404 (2014)
PMID: 25232738

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aggregative adherence fimbrial subunit AggA Chains: A, B
Molecule details ›
Chains: A, B
Length: 156 amino acids
Theoretical weight: 16.77 KDa
Source organism: Escherichia coli O104:H4 str. C227-11
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2221
Unit cell:
a: 77.832Å b: 80.172Å c: 91.416Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.156 0.232
Expression system: Escherichia coli BL21

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Citations

6 review citations

Classical chaperone-usher (CU) adhesive fimbriome: uropathogenic Escherichia coli (UPEC) and urinary tract infections (UTIs).
Behzadi P. (2020)
5 more

1 mention without citation

Structural insight into host recognition by aggregative adherence fimbriae of enteroaggregative Escherichia coli.
Berry et al. (2014)