› 4q9n

X-ray diffraction
1.8Å resolution

Crystal structure of Chlamydia trachomatis enoyl-ACP reductase (FabI) in complex with NADH and AFN-1252

Released:
DOI: 10.2210/pdb4q9n/pdb
PDB entry 4q9n coloured by chain, front view.
PDB entry 4q9n coloured by chain, side view.
PDB entry 4q9n coloured by chain, top view.
Source organism: Chlamydia trachomatis F/11-96
Primary publication:
Type II fatty acid synthesis is essential for the replication of Chlamydia trachomatis.
Yao J, Abdelrahman YM, Robertson RM, Cox JV, Belland RJ, White SW, Rock CO
J Biol Chem 289 22365-76 (2014)
PMID: 24958721

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-[acyl-carrier-protein] reductase [NADH] Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 298 amino acids
Theoretical weight: 31.99 KDa
Source organism: Chlamydia trachomatis F/11-96
Expression system: Escherichia coli BL21(DE3)
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand NAI 8 x NAI
Ligand 0WE 8 x 0WE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43
Unit cell:
a: 96.146Å b: 96.146Å c: 263.082Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Bacterial membrane lipids: diversity in structures and pathways.
Sohlenkamp et al. (2016)
8 more

4 mentions without citation

Type II fatty acid synthesis is essential for the replication of Chlamydia trachomatis.
Yao et al. (2014)
3 more