› 4qc3

X-ray diffraction
1.6Å resolution

Crystal structure of human BAZ2B bromodomain in complex with a diacetylated histone 4 peptide (H4K8acK12ac)

Released:
DOI: 10.2210/pdb4qc3/pdb
PDB entry 4qc3 coloured by chain, front view.
PDB entry 4qc3 coloured by chain, side view.
PDB entry 4qc3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
Tallant C, Valentini E, Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A
Structure 23 80-92 (2015)
PMID: 25533489

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-165092 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bromodomain adjacent to zinc finger domain protein 2B Chains: A, B
Molecule details ›
Chains: A, B
Length: 107 amino acids
Theoretical weight: 12.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UIF8 (Residues: 2062-2166; Coverage: 5%)
Gene names: BAZ2B, KIAA1476
Sequence domains: Bromodomain
Structure domains: Bromodomain-like
diacetylated histone 4 peptide (H4K8acK12ac) Chain: C
Molecule details ›
Chain: C
Length: 7 amino acids
Theoretical weight: 700 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EDO 3 x EDO
1 modified residue:
Ligand ALY 2 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 61.67Å b: 32.402Å c: 62.033Å
α: 90° β: 109.82° γ: 90°
R-values:
R R work R free
0.169 0.168 0.187
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

16 review citations

Mechanisms of action and regulation of ATP-dependent chromatin-remodelling complexes.
Clapier et al. (2017)
15 more

4 mentions without citation

Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
Tallant et al. (2015)
3 more