› 4qgo

X-ray diffraction
1.5Å resolution

Crystal structure of NucA from Streptococcus agalactiae with no metal bound

Released:
DOI: 10.2210/pdb4qgo/pdb
PDB entry 4qgo coloured by chain, front view.
PDB entry 4qgo coloured by chain, side view.
PDB entry 4qgo coloured by chain, top view.
Source organism: Streptococcus agalactiae ILRI112
Primary publication:
Structural characterization of the virulence factor nuclease A from Streptococcus agalactiae.
Moon AF, Gaudu P, Pedersen LC
Acta Crystallogr D Biol Crystallogr 70 2937-49 (2014)
PMID: 25372684

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165096 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-entry nuclease (Competence-specific nuclease) Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 25.24 KDa
Source organism: Streptococcus agalactiae ILRI112
Expression system: Escherichia coli
Structure domains: Extracellular Endonuclease, subunit A

Ligands and Environments

3 bound ligands:
Ligand SO4 9 x SO4
Ligand CL 4 x CL
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P1
Unit cell:
a: 37.591Å b: 57.131Å c: 62.422Å
α: 109.4° β: 97.08° γ: 90.16°
R-values:
R R work R free
0.154 0.153 0.18
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Pathogen-Derived Nucleases: An Effective Weapon for Escaping Extracellular Traps.
Liao et al. (2022)
4 other articles

2 mentions without citation

Structural characterization of the virulence factor nuclease A from Streptococcus agalactiae.
Moon et al. (2014)
1 more