› 4ql3

X-ray diffraction
1.04Å resolution

Crystal Structure of a GDP-bound G12R Oncogenic Mutant of Human GTPase KRas

Released:
DOI: 10.2210/pdb4ql3/pdb
PDB entry 4ql3 coloured by chain, front view.
PDB entry 4ql3 coloured by chain, side view.
PDB entry 4ql3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biochemical and Structural Analysis of Common Cancer-Associated KRAS Mutations.
Hunter JC, Manandhar A, Carrasco MA, Gurbani D, Gondi S, Westover KD
Mol Cancer Res 13 1325-35 (2015)
PMID: 26037647

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 19.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01116 (Residues: 1-169; Coverage: 89%)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 39.125Å b: 40.908Å c: 91.806Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.139 0.138 0.162
Expression system: Escherichia coli BL21

Quick links

Citations

114 review citations

RAS Proteins and Their Regulators in Human Disease.
Simanshu et al. (2017)
113 more

6 mentions without citation

Chemoselective Covalent Modification of K-Ras(G12R) with a Small Molecule Electrophile.
Zhang et al. (2022)
5 more