› 4qnw

X-ray diffraction
1.8Å resolution

Crystal structure of EasA, an old yellow enzyme from Aspergillus fumigatus

Released:
DOI: 10.2210/pdb4qnw/pdb
PDB entry 4qnw coloured by chain, front view.
PDB entry 4qnw coloured by chain, side view.
PDB entry 4qnw coloured by chain, top view.
Source organism: Aspergillus fumigatus Af293
Primary publication:
Structure of an Aspergillus fumigatus old yellow enzyme (EasA) involved in ergot alkaloid biosynthesis.
Chilton AS, Ellis AL, Lamb AL
Acta Crystallogr F Struct Biol Commun 70 1328-32 (2014)
PMID: 25286934

Function and Biology Details

Reaction catalysed: 
Dihydrochanoclavine-I aldehyde + NADP(+) = chanoclavine-I aldehyde + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175856 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chanoclavine-I aldehyde reductase easA Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 43.98 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4WZ70 (Residues: 1-376; Coverage: 100%)
Gene names: AFUA_2G17960, easA, fgaOx3
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
2 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 62.456Å b: 65.913Å c: 115.219Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.178 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Structural investigation into the C-terminal extension of the ene-reductase from Ralstonia (Cupriavidus) metallidurans.
Opperman DJ. (2017)

4 mentions without citation

Structure of an Aspergillus fumigatus old yellow enzyme (EasA) involved in ergot alkaloid biosynthesis.
Chilton et al. (2014)
3 more