4qpq

X-ray diffraction
3.11Å resolution

Mechanistic basis of plasmid-specific DNA binding of the F plasmid regulatory protein, TraM

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-118021 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Relaxosome protein TraM Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 53 amino acids
Theoretical weight: 6.02 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P10026 (Residues: 2-54; Coverage: 42%)
Gene names: ECOK12F071, traM
Sequence domains: TraM protein, DNA-binding
Structure domains: TraM protein, DNA-binding
sbmA DNA1 Chain: P
Molecule details ›
Chain: P
Length: 28 nucleotides
Theoretical weight: 8.66 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
sbmA DNA2 Chain: Q
Molecule details ›
Chain: Q
Length: 28 nucleotides
Theoretical weight: 8.55 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P43212
Unit cell:
a: 87.057Å b: 87.057Å c: 217.843Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.232
Expression systems:
  • Escherichia coli
  • Not provided