› 4qwp

X-ray diffraction
1.7Å resolution

co-crystal structure of chitosanase OU01 with substrate

Released:
DOI: 10.2210/pdb4qwp/pdb
PDB entry 4qwp coloured by chain, front view.
PDB entry 4qwp coloured by chain, side view.
PDB entry 4qwp coloured by chain, top view.
Source organism: Pseudomonas sp. A-01
Primary publication:
Structural and biochemical insights into the degradation mechanism of chitosan by chitosanase OU01.
Lyu Q, Shi Y, Wang S, Yang Y, Han B, Liu W, Jones DN, Liu W
Biochim Biophys Acta 1850 1953-61 (2015)
PMID: 26145578

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185228 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Chitosanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 26.56 KDa
Source organism: Pseudomonas sp. A-01
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KZM5 (Residues: 27-266; Coverage: 99%)
Sequence domains: Glycosyl hydrolase family 46
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GCS
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GCS
Carbohydrate polymer
2 bound ligands:
Ligand ACT 1 x ACT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 62.235Å b: 40.885Å c: 105.313Å
α: 90° β: 106.83° γ: 90°
R-values:
R R work R free
0.168 0.166 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes.
Viens et al. (2015)
3 more