› 4qyz

X-ray diffraction
3.03Å resolution

Crystal structure of a CRISPR RNA-guided surveillance complex, Cascade, bound to a ssDNA target

Released:
DOI: 10.2210/pdb4qyz/pdb
PDB entry 4qyz coloured by chain, front view.
PDB entry 4qyz coloured by chain, side view.
PDB entry 4qyz coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural biology. Crystal structure of a CRISPR RNA-guided surveillance complex bound to a ssDNA target.
Mulepati S, Héroux A, Bailey S
Science 345 1479-84 (2014)
PMID: 25123481

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118041 (preferred)
Entry contents:
5 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (7 distinct):
CRISPR system Cascade subunit CasA Chain: A
Molecule details ›
Chain: A
Length: 502 amino acids
Theoretical weight: 55.96 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46901 (Residues: 1-502; Coverage: 100%)
Gene names: JW2730, b2760, casA, cse1, ygcL
Sequence domains: CRISPR-associated protein Cse1 (CRISPR_cse1)
CRISPR system Cascade subunit CasB Chains: B, C
Molecule details ›
Chains: B, C
Length: 160 amino acids
Theoretical weight: 18.73 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P76632 (Residues: 1-160; Coverage: 100%)
Gene names: JW2729, b2759, casB, cse2, ygcK
Sequence domains: CRISPR-associated protein Cse2 (CRISPR_cse2)
Structure domains: CRISPR-associated protein Cse2
CRISPR system Cascade subunit CasC Chains: D, E, F, G, H, I
Molecule details ›
Chains: D, E, F, G, H, I
Length: 363 amino acids
Theoretical weight: 40.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46899 (Residues: 1-363; Coverage: 100%)
Gene names: JW2728, b2758, cas4, casC, cse4, ygcJ
Sequence domains: CT1975-like protein
CRISPR system Cascade subunit CasD Chain: J
Molecule details ›
Chain: J
Length: 224 amino acids
Theoretical weight: 25.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46898 (Residues: 1-224; Coverage: 100%)
Gene names: JW5844, b2757, cas5, casD, ygcI
Sequence domains: CRISPR-associated protein (Cas_Cas5)
Structure domains: Alpha-Beta Plaits
CRISPR system Cascade subunit CasE Chain: K
Molecule details ›
Chain: K
Length: 199 amino acids
Theoretical weight: 22.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46897 (Residues: 1-199; Coverage: 100%)
Gene names: JW2726, b2756, cas6e, casE, ygcH
Sequence domains: CRISPR associated protein
Structure domains: Crispr-associated protein; domain 1
DNA (33-MER) Chain: M
Molecule details ›
Chain: M
Length: 40 nucleotides
Theoretical weight: 12.19 KDa
RNA (55-MER) Chain: L
Molecule details ›
Chain: L
Length: 61 nucleotides
Theoretical weight: 19.73 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P3121
Unit cell:
a: 223.869Å b: 223.869Å c: 290.651Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.224 0.267
Expression system: Escherichia coli

Quick links

Citations

29 review citations

An updated evolutionary classification of CRISPR-Cas systems.
Makarova et al. (2015)
28 more

14 mentions without citation

The structural biology of CRISPR-Cas systems.
Jiang et al. (2015)
13 more