› 4r3o

X-ray diffraction
2.6Å resolution

Human Constitutive 20S Proteasome

Released:
DOI: 10.2210/pdb4r3o/pdb
PDB entry 4r3o coloured by chain, front view.
PDB entry 4r3o coloured by chain, side view.
PDB entry 4r3o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human 20S proteasome in complex with carfilzomib.
Harshbarger W, Miller C, Diedrich C, Sacchettini J
Structure 23 418-24 (2015)
PMID: 25599644

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127173 (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Proteasome subunit alpha type-6 Chains: A, O
Molecule details ›
Chains: A, O
Length: 244 amino acids
Theoretical weight: 27.19 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60900 (Residues: 2-245; Coverage: 99%)
Gene names: PROS27, PSMA6
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-2 Chains: B, P
Molecule details ›
Chains: B, P
Length: 233 amino acids
Theoretical weight: 25.8 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25787 (Residues: 2-234; Coverage: 100%)
Gene names: HC3, PSC3, PSMA2
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-4 Chains: C, Q
Molecule details ›
Chains: C, Q
Length: 250 amino acids
Theoretical weight: 28.12 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25789 (Residues: 2-251; Coverage: 96%)
Gene names: HC9, PSC9, PSMA4
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-7 Chains: D, R
Molecule details ›
Chains: D, R
Length: 243 amino acids
Theoretical weight: 27.38 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O14818 (Residues: 2-244; Coverage: 98%)
Gene names: HSPC, PSMA7
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-5 Chains: E, S
Molecule details ›
Chains: E, S
Length: 234 amino acids
Theoretical weight: 25.57 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28066 (Residues: 8-241; Coverage: 97%)
Gene name: PSMA5
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-1 Chains: F, T
Molecule details ›
Chains: F, T
Length: 238 amino acids
Theoretical weight: 26.73 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25786 (Residues: 4-241; Coverage: 91%)
Gene names: HC2, NU, PROS30, PSC2, PSMA1
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-3 Chains: G, U
Molecule details ›
Chains: G, U
Length: 245 amino acids
Theoretical weight: 27.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25788 (Residues: 2-246; Coverage: 96%)
Gene names: HC8, PSC8, PSMA3
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-6 Chains: H, V
Molecule details ›
Chains: H, V
Length: 202 amino acids
Theoretical weight: 21.66 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28072 (Residues: 35-236; Coverage: 85%)
Gene names: LMPY, PSMB6, Y
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-7 Chains: I, W
Molecule details ›
Chains: I, W
Length: 220 amino acids
Theoretical weight: 23.75 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q99436 (Residues: 44-263; Coverage: 79%)
Gene names: PSMB7, Z
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-3 Chains: J, X
Molecule details ›
Chains: J, X
Length: 204 amino acids
Theoretical weight: 22.84 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49720 (Residues: 2-205; Coverage: 100%)
Gene name: PSMB3
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-2 Chains: K, Y
Molecule details ›
Chains: K, Y
Length: 199 amino acids
Theoretical weight: 22.72 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49721 (Residues: 1-199; Coverage: 99%)
Gene name: PSMB2
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-5 Chains: L, Z
Molecule details ›
Chains: L, Z
Length: 201 amino acids
Theoretical weight: 22.2 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28074 (Residues: 60-260; Coverage: 76%)
Gene names: LMPX, MB1, PSMB5, X
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-1 Chains: 1, M
Molecule details ›
Chains: 1, M
Length: 213 amino acids
Theoretical weight: 23.58 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P20618 (Residues: 29-241; Coverage: 88%)
Gene names: PSC5, PSMB1
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-4 Chains: 2, N
Molecule details ›
Chains: 2, N
Length: 217 amino acids
Theoretical weight: 24.14 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28070 (Residues: 46-262; Coverage: 82%)
Gene names: PROS26, PSMB4
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 119.227Å b: 205.061Å c: 163.128Å
α: 90° β: 106.37° γ: 90°
R-values:
R R work R free
0.206 0.204 0.244

Quick links

Citations

18 review citations

The Proteasome in Modern Drug Discovery: Second Life of a Highly Valuable Drug Target.
Cromm et al. (2017)
17 more

22 mentions without citation

A Practical Review of Proteasome Pharmacology.
Thibaudeau et al. (2019)
21 more