› 4r79

X-ray diffraction
3.1Å resolution

Mos1 transposase paired-end complex with left transposon end

Released:
DOI: 10.2210/pdb4r79/pdb
PDB entry 4r79 coloured by chain, front view.
PDB entry 4r79 coloured by chain, side view.
PDB entry 4r79 coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for the Inverted Repeat Preferences of mariner Transposases.
Trubitsyna M, Grey H, Houston DR, Finnegan DJ, Richardson JM
J Biol Chem 290 13531-40 (2015)
PMID: 25869132

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118080 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Mariner Mos1 transposase Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 40.87 KDa
Source organism: Drosophila mauritiana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7JQ07 (Residues: 1-345; Coverage: 100%)
Gene name: mariner\T
Sequence domains:
Structure domains:
left Inverted repeat NTS Chains: C, E, G
Molecule details ›
Chains: C, E, G
Length: 25 nucleotides
Theoretical weight: 7.83 KDa
Source organism: synthetic construct
Expression system: Not provided
left Inverted repeat TS Chains: D, F
Molecule details ›
Chains: D, F
Length: 28 nucleotides
Theoretical weight: 8.49 KDa
Source organism: synthetic construct
Expression system: Not provided
left Inverted repeat NTS H Chain: H
Molecule details ›
Chain: H
Length: 27 nucleotides
Theoretical weight: 8.16 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 119.783Å b: 86.885Å c: 132.379Å
α: 90° β: 99.03° γ: 90°
R-values:
R R work R free
0.229 0.227 0.27
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

DNA Transposition at Work.
Hickman et al. (2016)
5 other articles

3 mentions without citation

C. elegans as a model for membrane traffic.
Sato et al. (2014)
2 more