› 4ray

X-ray diffraction
1.55Å resolution

Crystal structure of Magnetospirillum gryphiswaldense MSR-1 Apo-Fur

Released:
DOI: 10.2210/pdb4ray/pdb
PDB entry 4ray coloured by chain, front view.
PDB entry 4ray coloured by chain, side view.
PDB entry 4ray coloured by chain, top view.
Source organism: Magnetospirillum gryphiswaldense MSR-1 v2
Primary publication:
Mechanistic insights into metal ion activation and operator recognition by the ferric uptake regulator.
Deng Z, Wang Q, Liu Z, Zhang M, Machado AC, Chiu TP, Feng C, Zhang Q, Yu L, Qi L, Zheng J, Wang X, Huo X, Qi X, Li X, Wu W, Rohs R, Li Y, Chen Z
Nat Commun 6 7642 (2015)
PMID: 26134419

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-197674 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferric uptake regulation protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 16.67 KDa
Source organism: Magnetospirillum gryphiswaldense MSR-1 v2
Expression system: Escherichia coli
UniProt:
  • Canonical: V6F4Q0 (Residues: 1-143; Coverage: 100%)
Gene names: Fur, MGMSRv2__3137, fur
Sequence domains: Ferric uptake regulator family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FLC 1 x FLC
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 69.192Å b: 78.151Å c: 66.316Å
α: 90° β: 108.47° γ: 90°
R-values:
R R work R free
0.185 0.183 0.208
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Metal homeostasis and resistance in bacteria.
Chandrangsu et al. (2017)
12 more

6 mentions without citation

Bacterial iron detoxification at the molecular level.
Bradley et al. (2020)
5 more