› 4rkg

X-ray diffraction
2.5Å resolution

Structure of the MSL2 CXC domain bound with a non-specific (GC)6 DNA

Released:
DOI: 10.2210/pdb4rkg/pdb
PDB entry 4rkg coloured by chain, front view.
PDB entry 4rkg coloured by chain, side view.
PDB entry 4rkg coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural basis of X chromosome DNA recognition by the MSL2 CXC domain during Drosophila dosage compensation.
Zheng S, Villa R, Wang J, Feng Y, Wang J, Becker PB, Ye K
Genes Dev 28 2652-62 (2014)
PMID: 25452275

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118105 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase msl-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 52 amino acids
Theoretical weight: 5.65 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P50534 (Residues: 520-570; Coverage: 7%)
Gene names: CG3241, msl-2
Sequence domains: CXC domain of E3 ubiquitin-protein ligase MSL2
DNA (5'-D(*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP*GP*C)-3') Chains: H, I
Molecule details ›
Chains: H, I
Length: 12 nucleotides
Theoretical weight: 3.67 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 9 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 40.705Å b: 75.251Å c: 77.436Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.226 0.315
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Dosage Compensation in Drosophila-a Model for the Coordinate Regulation of Transcription.
Kuroda et al. (2016)
4 more

1 mention without citation

Structural basis of X chromosome DNA recognition by the MSL2 CXC domain during Drosophila dosage compensation.
Zheng et al. (2014)