› 4rkh

X-ray diffraction
2Å resolution

Structure of the MSL2 CXC domain bound with a specific MRE sequence

Released:
DOI: 10.2210/pdb4rkh/pdb
PDB entry 4rkh coloured by chain, front view.
PDB entry 4rkh coloured by chain, side view.
PDB entry 4rkh coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural basis of X chromosome DNA recognition by the MSL2 CXC domain during Drosophila dosage compensation.
Zheng S, Villa R, Wang J, Feng Y, Wang J, Becker PB, Ye K
Genes Dev 28 2652-62 (2014)
PMID: 25452275

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118106 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
E3 ubiquitin-protein ligase msl-2 Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 52 amino acids
Theoretical weight: 5.65 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P50534 (Residues: 520-570; Coverage: 7%)
Gene names: CG3241, msl-2
Sequence domains: CXC domain of E3 ubiquitin-protein ligase MSL2
DNA (5'-D(*AP*TP*GP*AP*GP*CP*GP*AP*GP*AP*TP*GP*GP*AP*T)-3') Chain: A
Molecule details ›
Chain: A
Length: 15 nucleotides
Theoretical weight: 4.7 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
DNA (5'-D(*AP*TP*CP*CP*AP*TP*CP*TP*CP*GP*CP*TP*CP*AP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.48 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 49.371Å b: 50.998Å c: 124.934Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.254
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Dosage Compensation of the X Chromosome: A Complex Epigenetic Assignment Involving Chromatin Regulators and Long Noncoding RNAs.
Samata et al. (2018)
4 more

2 mentions without citation

Structural basis for LIN54 recognition of CHR elements in cell cycle-regulated promoters.
Marceau et al. (2016)
1 more