› 4rqo

X-ray diffraction
2.25Å resolution

Crystal structure of L-Serine Dehydratase from Legionella pneumophila

Released:
DOI: 10.2210/pdb4rqo/pdb
PDB entry 4rqo coloured by chain, front view.
PDB entry 4rqo coloured by chain, side view.
PDB entry 4rqo coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Thunder Bay
Primary publication:
Structure of L-serine dehydratase from Legionella pneumophila: novel use of the C-terminal cysteine as an intrinsic competitive inhibitor.
Thoden JB, Holden HM, Grant GA
Biochemistry 53 7615-24 (2014)
PMID: 25380533

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-serine dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 476 amino acids
Theoretical weight: 52.6 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Thunder Bay
Expression system: Escherichia coli BL21(DE3)
Structure domains: D-3-phosphoglycerate dehydrogenase; domain 3

Ligands and Environments

2 bound ligands:
Ligand SF4 2 x SF4
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3121
Unit cell:
a: 81.39Å b: 81.39Å c: 267.521Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.198 0.258
Expression system: Escherichia coli BL21(DE3)

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Citations

5 citation in other articles

Gene sdaB Is Involved in the Nematocidal Activity of Enterobacter ludwigii AA4 Against the Pine Wood Nematode Bursaphelenchus xylophilus.
Zhao et al. (2022)
4 more

3 mentions without citation

Hydrogen-Deuterium Exchange Mass Spectrometry Reveals Unique Conformational and Chemical Transformations Occurring upon [4Fe-4S] Cluster Binding in the Type 2 L-Serine Dehydratase from Legionella pneumophila.
Yan et al. (2015)
2 more