› 4rs5

X-ray diffraction
3.8Å resolution

Crystal structure of an uncoating intermediate of a EV71 recombinant virus

Released:
DOI: 10.2210/pdb4rs5/pdb
PDB entry 4rs5 coloured by chain, front view.
PDB entry 4rs5 coloured by chain, side view.
PDB entry 4rs5 coloured by chain, top view.
Source organism: Enterovirus A71
Primary publication:
Crystal structures of enterovirus 71 (EV71) recombinant virus particles provide insights into vaccine design.
Lyu K, Wang GC, He YL, Han JF, Ye Q, Qin CF, Chen R
J Biol Chem 290 3198-208 (2015)
PMID: 25492868

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 180-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123621 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Capsid protein VP3 Chains: B, E, H, K, N
Molecule details ›
Chains: B, E, H, K, N
Length: 242 amino acids
Theoretical weight: 26.44 KDa
Source organism: Enterovirus A71
Expression system: Homo sapiens
UniProt:
  • Canonical: F6KTB0 (Residues: 324-565; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP0 Chains: C, F, I, L, O
Molecule details ›
Chains: C, F, I, L, O
Length: 323 amino acids
Theoretical weight: 35.21 KDa
Source organism: Enterovirus A71
Expression system: Homo sapiens
UniProt:
  • Canonical: F6KTB0 (Residues: 1-323; Coverage: 15%)
Sequence domains:
Structure domains: Jelly Rolls
Capsid protein VP1 Chains: A, D, G, J, M
Molecule details ›
Chains: A, D, G, J, M
Length: 313 amino acids
Theoretical weight: 34.37 KDa
Source organism: Enterovirus A71
Expression system: Homo sapiens
UniProt:
  • Canonical: F6KTB0 (Residues: 566-665, 666-862; Coverage: 14%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P4232
Unit cell:
a: 350.155Å b: 350.155Å c: 350.155Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.27 0.299
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Is a multivalent hand, foot, and mouth disease vaccine feasible?
Klein et al. (2015)
6 more

1 mention without citation

Crystal structures of enterovirus 71 (EV71) recombinant virus particles provide insights into vaccine design.
Lyu et al. (2015)