› 4rsu

X-ray diffraction
2.3Å resolution

Crystal structure of the light and hvem complex

Released:
DOI: 10.2210/pdb4rsu/pdb
PDB entry 4rsu coloured by chain, front view.
PDB entry 4rsu coloured by chain, side view.
PDB entry 4rsu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
HVEM structures and mutants reveal distinct functions of binding to LIGHT and BTLA/CD160.
Liu W, Chou TF, Garrett-Thomson SC, Seo GY, Fedorov E, Ramagopal UA, Bonanno JB, Wang Q, Kim K, Garforth SJ, Kakugawa K, Cheroutre H, Kronenberg M, Almo SC
J Exp Med 218 (2021)
PMID: 34709351

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dodecamer
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128993 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor necrosis factor ligand superfamily member 14, soluble form Chains: A, B, C, G, H, I
Molecule details ›
Chains: A, B, C, G, H, I
Length: 165 amino acids
Theoretical weight: 18.19 KDa
Source organism: Homo sapiens
Expression system: Drosophila
UniProt:
  • Canonical: O43557 (Residues: 83-240; Coverage: 66%)
Gene names: HVEML, LIGHT, TNFSF14, UNQ391/PRO726
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
Tumor necrosis factor receptor superfamily member 14 Chains: D, E, F, J, K, L
Molecule details ›
Chains: D, E, F, J, K, L
Length: 134 amino acids
Theoretical weight: 14.33 KDa
Source organism: Homo sapiens
Expression system: Drosophila
UniProt:
  • Canonical: Q92956 (Residues: 39-162; Coverage: 51%)
Gene names: HVEA, HVEM, TNFRSF14, UNQ329/PRO509
Sequence domains: TNFR/NGFR cysteine-rich region
Structure domains: Tumor Necrosis Factor Receptor, subunit A, domain 2

Ligands and Environments

3 bound ligands:
Ligand CL 14 x CL
Ligand GOL 8 x GOL
Ligand NAG 5 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 111.651Å b: 113.604Å c: 163.279Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.231
Expression system: Drosophila

Quick links

Citations

4 review citations

MicroRNAs with Multiple Targets of Immune Checkpoints, as a Potential Sensitizer for Immune Checkpoint Inhibitors in Breast Cancer Treatment.
Zhou et al. (2023)
3 more

7 mentions without citation

On the TRAIL of Better Therapies: Understanding TNFRSF Structure-Function.
Vanamee et al. (2020)
6 more