› 4rub

X-ray diffraction
2.7Å resolution

A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE

Released:
DOI: 10.2210/pdb4rub/pdb
PDB entry 4rub coloured by chain, front view.
PDB entry 4rub coloured by chain, side view.
PDB entry 4rub coloured by chain, top view.
Source organism: Nicotiana tabacum
Primary publication:
A crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase from Nicotiana tabacum in the activated state.
Suh SW, Cascio D, Chapman MS, Eisenberg D
J Mol Biol 197 363-5 (1987)
PMID: 3681999

Function and Biology Details

Reaction catalysed: 
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133653 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribulose bisphosphate carboxylase large chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 477 amino acids
Theoretical weight: 52.97 KDa
Source organism: Nicotiana tabacum
Expression system: Not provided
UniProt:
  • Canonical: P00876 (Residues: 1-477; Coverage: 100%)
Gene names: rbcA, rbcL
Sequence domains:
Structure domains:
Ribulose bisphosphate carboxylase small subunit, chloroplastic Chains: S, T, U, V
Molecule details ›
Chains: S, T, U, V
Length: 123 amino acids
Theoretical weight: 14.5 KDa
Source organism: Nicotiana tabacum
Expression system: Not provided
UniProt:
  • Canonical: P69249 (Residues: 58-180; Coverage: 68%)
Gene name: RBCS
Sequence domains: Ribulose bisphosphate carboxylase, small chain
Structure domains: Ribulose bisphosphate carboxylase, small subunit

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand CAP 4 x CAP
Ligand FMT 4 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 204.6Å b: 204.6Å c: 117.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 not available not available
Expression system: Not provided

Quick links

Citations

3 review citations

Biogenesis and Metabolic Maintenance of Rubisco.
Bracher et al. (2017)
2 more

10 mentions without citation

Conformational changes in redox pairs of protein structures.
Fan et al. (2009)
9 more