› 4s3l

X-ray diffraction
2.8Å resolution

Crystal Structure of major pilin protein PitB from type II pilus of Streptococcus pneumoniae

Released:
DOI: 10.2210/pdb4s3l/pdb
PDB entry 4s3l coloured by chain, front view.
PDB entry 4s3l coloured by chain, side view.
PDB entry 4s3l coloured by chain, top view.
Source organism: Streptococcus pneumoniae Taiwan19F-14
Primary publication:
A structural snapshot of type II pilus formation in Streptococcus pneumoniae.
Shaik MM, Lombardi C, Maragno Trindade D, Fenel D, Schoehn G, Di Guilmi AM, Dessen A
J Biol Chem 290 22581-92 (2015)
PMID: 26198632

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109852 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF5979 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 341 amino acids
Theoretical weight: 37.2 KDa
Source organism: Streptococcus pneumoniae Taiwan19F-14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B3FNT1 (Residues: 46-386; Coverage: 83%)
Gene name: pitB
Sequence domains: Domain of unknown function (DUF5979)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4, ESRF BEAMLINE BM14
Spacegroup: P43
Unit cell:
a: 54.21Å b: 54.21Å c: 361.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.239 0.298
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Sortase Transpeptidases: Structural Biology and Catalytic Mechanism.
Jacobitz et al. (2017)
2 more

3 mentions without citation

Pilus biogenesis of Gram-positive bacteria: Roles of sortases and implications for assembly.
Khare et al. (2017)
2 more