› 4tkd

X-ray diffraction
2.01Å resolution

Sulfolobus solfataricus HJC mutants

Released:
DOI: 10.2210/pdb4tkd/pdb
PDB entry 4tkd coloured by chain, front view.
PDB entry 4tkd coloured by chain, side view.
PDB entry 4tkd coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
Crystal Unengineering: Reducing the Crystallisability of Sulfolobus solfataricus Hjc
Middleton CL, Parker JL, Knott GJ, White MF, Bond CS
Aust. J. Chem. 67 1818-1823 (2014)

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181674 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Crossover junction endodeoxyribonuclease Hjc Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 141 amino acids
Theoretical weight: 15.8 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7LXU0 (Residues: 1-143; Coverage: 99%)
Gene names: ORF-c21_024, SSO0575, hjc
Sequence domains: Archaeal holliday junction resolvase (hjc)
Structure domains: Trna Endonuclease; Chain: A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 64.601Å b: 61.834Å c: 55.245Å
α: 90° β: 95.74° γ: 90°
R-values:
R R work R free
0.197 0.194 0.246
Expression system: Escherichia coli

Quick links