› 4tkk

X-ray diffraction
2.4Å resolution

Sulfolobus solfataricus HJC mutants

Released:
DOI: 10.2210/pdb4tkk/pdb
PDB entry 4tkk coloured by chain, front view.
PDB entry 4tkk coloured by chain, side view.
PDB entry 4tkk coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
Crystal Unengineering: Reducing the Crystallisability of Sulfolobus solfataricus Hjc
Middleton CL, Parker JL, Knott GJ, White MF, Bond CS
Aust. J. Chem. 67 1818-1823 (2014)

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181673 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Crossover junction endodeoxyribonuclease Hjc Chains: A, B
Molecule details ›
Chains: A, B
Length: 143 amino acids
Theoretical weight: 16.02 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7LXU0 (Residues: 1-143; Coverage: 100%)
Gene names: ORF-c21_024, SSO0575, hjc
Sequence domains: Archaeal holliday junction resolvase (hjc)
Structure domains: Trna Endonuclease; Chain: A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: I23
Unit cell:
a: 141.172Å b: 141.172Å c: 141.172Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.248
Expression system: Escherichia coli

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