› 4toq

X-ray diffraction
1.6Å resolution

Crystal structure of class III chitinase from pomegranate provides the insight into its metal storage capacity

Released:
DOI: 10.2210/pdb4toq/pdb
PDB entry 4toq coloured by chain, front view.
PDB entry 4toq coloured by chain, side view.
PDB entry 4toq coloured by chain, top view.
Source organism: Punica granatum
Primary publication:
Crystal structure of class III chitinase from pomegranate provides the insight into its metal storage capacity.
Masuda T, Zhao G, Mikami B
Biosci Biotechnol Biochem 79 45-50 (2015)
PMID: 25252615

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124237 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acidic endochitinase Pun g 14, amyloplastic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 273 amino acids
Theoretical weight: 29.03 KDa
Source organism: Punica granatum
Expression system: Escherichia coli
UniProt:
  • Canonical: G1UH28 (Residues: 27-299; Coverage: 91%)
Gene names: CDL15_Pgr011527, PSC
Sequence domains: Glycosyl hydrolases family 18
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand MG 13 x MG
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 58.462Å b: 79.969Å c: 97.532Å
α: 90° β: 102.97° γ: 90°
R-values:
R R work R free
0.157 0.155 0.192
Expression system: Escherichia coli

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Citations

7 citation in other articles

Structure, Catalysis, and Inhibition of OfChi-h, the Lepidoptera-exclusive Insect Chitinase.
Liu et al. (2017)
6 more

1 mention without citation

Chitinases as Food Allergens.
Leoni et al. (2019)