› 4tr8

X-ray diffraction
1.8Å resolution

Crystal structure of DNA polymerase sliding clamp from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb4tr8/pdb
PDB entry 4tr8 coloured by chain, front view.
PDB entry 4tr8 coloured by chain, side view.
PDB entry 4tr8 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa HB15
Primary publication:
Differential modes of peptide binding onto replicative sliding clamps from various bacterial origins.
Wolff P, Amal I, Oliéric V, Chaloin O, Gygli G, Ennifar E, Lorber B, Guichard G, Wagner J, Dejaegere A, Burnouf DY
J Med Chem 57 7565-76 (2014)
PMID: 25170813

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta sliding clamp Chains: A, B
Molecule details ›
Chains: A, B
Length: 383 amino acids
Theoretical weight: 42.55 KDa
Source organism: Pseudomonas aeruginosa HB15
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I7C4 (Residues: 1-367; Coverage: 100%)
Gene names: PA0002, dnaN
Sequence domains:
Structure domains: DNA Polymerase III, subunit A, domain 2

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 80.53Å b: 92.41Å c: 127.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.232
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Recent advancements in the development of anti-tuberculosis drugs.
Chetty et al. (2017)
3 more

1 mention without citation

Structural insight into β-Clamp and its interaction with DNA Ligase in Helicobacter pylori.
Pandey et al. (2016)