› 4ttb

X-ray diffraction
2.45Å resolution

Crystal structure of homo sapiens IODOTYROSINE DEIODINASE (IYD) bound to FMN

Released:
DOI: 10.2210/pdb4ttb/pdb
PDB entry 4ttb coloured by chain, front view.
PDB entry 4ttb coloured by chain, side view.
PDB entry 4ttb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A switch between one- and two-electron chemistry of the human flavoprotein iodotyrosine deiodinase is controlled by substrate.
Hu J, Chuenchor W, Rokita SE
J Biol Chem 290 590-600 (2015)
PMID: 25395621

Function and Biology Details

Reaction catalysed: 
(1a) L-tyrosine + NADP(+) + iodide = 3-iodo-L-tyrosine + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iodotyrosine deiodinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 30.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6PHW0 (Residues: 32-289; Coverage: 89%)
Gene names: C6orf71, DEHAL1, IYD
Sequence domains: Nitroreductase family
Structure domains: NADH Oxidase

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P31
Unit cell:
a: 72.892Å b: 72.892Å c: 95.515Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.193 0.203
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse.
Agarwal et al. (2017)
7 more

2 mentions without citation

The distribution and mechanism of iodotyrosine deiodinase defied expectations.
Sun et al. (2017)
1 more