› 4tug

X-ray diffraction
3.55Å resolution

Crystal structure of MjMre11-DNA2 complex

Released:
DOI: 10.2210/pdb4tug/pdb
PDB entry 4tug coloured by chain, front view.
PDB entry 4tug coloured by chain, side view.
PDB entry 4tug coloured by chain, top view.
Source organisms:
Primary publication:
DNA end recognition by the Mre11 nuclease dimer: insights into resection and repair of damaged DNA.
Sung S, Li F, Park YB, Kim JS, Kim AK, Song OK, Kim J, Che J, Lee SE, Cho Y
EMBO J 33 2422-35 (2014)
PMID: 25107472

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118167 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA double-strand break repair protein Mre11 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 337 amino acids
Theoretical weight: 39.7 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58719 (Residues: 1-333; Coverage: 91%)
Gene names: MJ1323, mre11
Sequence domains: Calcineurin-like phosphoesterase
Structure domains:
DNA (5'-D(P*GP*CP*AP*CP*GP*TP*AP*GP*GP*AP*CP*AP*GP*C)-3') Chain: G
Molecule details ›
Chain: G
Length: 14 nucleotides
Theoretical weight: 4.31 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*CP*TP*GP*TP*CP*CP*TP*AP*CP*GP*TP*GP*CP*CP*A)-3') Chain: H
Molecule details ›
Chain: H
Length: 15 nucleotides
Theoretical weight: 4.52 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 12 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 91.53Å b: 185.624Å c: 106.212Å
α: 90° β: 99.86° γ: 90°
R-values:
R R work R free
0.187 0.184 0.236
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

The MRE11-RAD50-NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair.
Syed et al. (2018)
9 more

4 mentions without citation

Functional and structural insights into the MRX/MRN complex, a key player in recognition and repair of DNA double-strand breaks.
Tisi et al. (2020)
3 more