› 4tvs

X-ray diffraction
1.6Å resolution

LAP1(aa356-583), H.sapiens, bound to VHH-BS1

Released:
DOI: 10.2210/pdb4tvs/pdb
PDB entry 4tvs coloured by chain, front view.
PDB entry 4tvs coloured by chain, side view.
PDB entry 4tvs coloured by chain, top view.
Source organisms:
Primary publication:
How lamina-associated polypeptide 1 (LAP1) activates Torsin.
Sosa BA, Demircioglu FE, Chen JZ, Ingram J, Ploegh HL, Schwartz TU
Elife 3 e03239 (2014)
PMID: 25149450

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230366 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Torsin-1A-interacting protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 231 amino acids
Theoretical weight: 26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JTV8 (Residues: 356-583; Coverage: 39%)
Gene names: LAP1, TOR1AIP1
Sequence domains: Lamina-associated polypeptide 1, AAA+ activator domain
Structure domains: Rossmann fold
VHH Domain BS-1 Chains: a, b
Molecule details ›
Chains: a, b
Length: 134 amino acids
Theoretical weight: 14.69 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 69.786Å b: 74.021Å c: 85.425Å
α: 90° β: 108.79° γ: 90°
R-values:
R R work R free
0.182 0.181 0.229
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Mechanisms and functions of nuclear envelope remodelling.
Ungricht et al. (2017)
17 more

8 mentions without citation

Torsins: not your typical AAA+ ATPases.
Rose et al. (2015)
7 more