› 4tx7

X-ray diffraction
1.55Å resolution

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190370 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
chitinase Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 26.55 KDa
Source organism: Vigna unguiculata subsp. sesquipedalis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9FUH3 (Residues: 43-287; Coverage: 83%)
Sequence domains: Chitinase class I
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P3121
Unit cell:
a: 69.32Å b: 69.32Å c: 114.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.15 0.149 0.173
Expression system: Komagataella pastoris

Quick links

Citations

4 review citations

Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins.
Karbalaei et al. (2020)
3 more

3 mentions without citation

Structural Analysis and Construction of a Thermostable Antifungal Chitinase.
Kozome et al. (2022)
2 more