› 4tyv

X-ray diffraction
1.75Å resolution

Ensemble refinement of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with glucose

Released:
DOI: 10.2210/pdb4tyv/pdb
PDB entry 4tyv coloured by chain, ensemble of 25 models, front view.
PDB entry 4tyv coloured by chain, ensemble of 25 models, side view.
PDB entry 4tyv coloured by chain, ensemble of 25 models, top view.
Source organism: Streptomyces sp. SirexAA-E
Primary publication:
Active site and laminarin binding in glycoside hydrolase family 55.
Bianchetti CM, Takasuka TE, Deutsch S, Udell HS, Yik EJ, Bergeman LF, Fox BG
J Biol Chem 290 11819-32 (2015)
PMID: 25752603

Function and Biology Details

Reaction catalysed: 
Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124265 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exo-beta-1,3-glucanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 551 amino acids
Theoretical weight: 59.08 KDa
Source organism: Streptomyces sp. SirexAA-E
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G2NFJ9 (Residues: 55-605; Coverage: 98%)
Gene name: SACTE_4363

Ligands and Environments

2 bound ligands:
Ligand EDO 5 x EDO
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 53.939Å b: 100.055Å c: 99.556Å
α: 90° β: 92.31° γ: 90°
R-values:
R R work R free
0.112 0.11 0.143
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Identifying and Visualizing Macromolecular Flexibility in Structural Biology.
Palamini et al. (2016)
2 more

1 mention without citation

Active site and laminarin binding in glycoside hydrolase family 55.
Bianchetti et al. (2015)