› 4tyw

X-ray diffraction
2.2Å resolution

DEAD-box helicase Mss116 bound to ssRNA and ADP-BeF

Released:
DOI: 10.2210/pdb4tyw/pdb
PDB entry 4tyw coloured by chain, front view.
PDB entry 4tyw coloured by chain, side view.
PDB entry 4tyw coloured by chain, top view.
Source organisms:
Primary publication:
Molecular insights into RNA and DNA helicase evolution from the determinants of specificity for a DEAD-box RNA helicase.
Mallam AL, Sidote DJ, Lambowitz AM
Elife 3 e04630 (2014)
PMID: 25497230

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147357 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
ATP-dependent RNA helicase MSS116, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 509 amino acids
Theoretical weight: 57.79 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P15424 (Residues: 88-595; Coverage: 77%)
Gene names: MSS116, YD9346.05C, YDR194C
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 7 nucleotides
Theoretical weight: 2.26 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand BEF 1 x BEF
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21212
Unit cell:
a: 89.829Å b: 126.26Å c: 55.545Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.254
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Distinct RNA-unwinding mechanisms of DEAD-box and DEAH-box RNA helicase proteins in remodeling structured RNAs and RNPs.
Gilman et al. (2017)
4 more

2 mentions without citation

Molecular insights into RNA and DNA helicase evolution from the determinants of specificity for a DEAD-box RNA helicase.
Mallam et al. (2014)
1 more