4u7b

X-ray diffraction
3.09Å resolution

Crystal structure of a pre-cleavage Mos1 transpososome

Released:
Source organism: Drosophila mauritiana
Primary publication:
Structural role of the flanking DNA in mariner transposon excision.
Nucleic Acids Res 43 2424-32 (2015)
PMID: 25662605

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-118181 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Mariner Mos1 transposase Chains: A, B, G
Molecule details ›
Chains: A, B, G
Length: 342 amino acids
Theoretical weight: 40.52 KDa
Source organism: Drosophila mauritiana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7JQ07 (Residues: 4-345; Coverage: 99%)
Gene name: mariner\T
Sequence domains:
Structure domains:
DNA (25-MER) Chains: C, E, H
Molecule details ›
Chains: C, E, H
Length: 25 nucleotides
Theoretical weight: 7.77 KDa
Source organism: Drosophila mauritiana
Expression system: Not provided
DNA (31-MER) Chains: D, F, I
Molecule details ›
Chains: D, F, I
Length: 31 nucleotides
Theoretical weight: 9.48 KDa
Source organism: Drosophila mauritiana
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 96.471Å b: 340.09Å c: 160.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.261
Expression systems:
  • Escherichia coli
  • Not provided