› 4u7y

X-ray diffraction
2.5Å resolution

Structure of the complex of VPS4B MIT and IST1 MIM

Released:
DOI: 10.2210/pdb4u7y/pdb
PDB entry 4u7y coloured by chain, front view.
PDB entry 4u7y coloured by chain, side view.
PDB entry 4u7y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Distinct mechanisms of recognizing endosomal sorting complex required for transport III (ESCRT-III) protein IST1 by different microtubule interacting and trafficking (MIT) domains.
Guo EZ, Xu Z
J Biol Chem 290 8396-408 (2015)
PMID: 25657007

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130909 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vacuolar protein sorting-associated protein 4B Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 10.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75351 (Residues: 1-89; Coverage: 20%)
Gene names: MIG1, SKD1, VPS42, VPS4B
Sequence domains: MIT (microtubule interacting and transport) domain
Structure domains: Phosphotransferase system, lactose/cellobiose-type IIA subunit
IST1 homolog Chain: B
Molecule details ›
Chain: B
Length: 25 amino acids
Theoretical weight: 3.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53990 (Residues: 341-364; Coverage: 7%)
Gene names: IST1, KIAA0174

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P622
Unit cell:
a: 78.401Å b: 78.401Å c: 67.642Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.225 0.271
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Cellular Functions and Molecular Mechanisms of the ESCRT Membrane-Scission Machinery.
Christ et al. (2017)
7 more

2 mentions without citation

Distinct mechanisms of recognizing endosomal sorting complex required for transport III (ESCRT-III) protein IST1 by different microtubule interacting and trafficking (MIT) domains.
Guo et al. (2015)
1 more