› 4uam

X-ray diffraction
1.8Å resolution

1.8 Angstrom crystal structure of IMP-1 metallo-beta-lactamase with a mixed iron-zinc center in the active site

Released:
DOI: 10.2210/pdb4uam/pdb
PDB entry 4uam coloured by chain, front view.
PDB entry 4uam coloured by chain, side view.
PDB entry 4uam coloured by chain, top view.
Source organism: Pseudomonas aeruginosa NCGM2.S1
Primary publication:
Iron(III) located in the dinuclear metallo-β-lactamase IMP-1 by pseudocontact shifts.
Carruthers TJ, Carr PD, Loh CT, Jackson CJ, Otting G
Angew Chem Int Ed Engl 53 14269-72 (2014)
PMID: 25320022

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165211 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
IMP-1 metallo-beta-lactamase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 228 amino acids
Theoretical weight: 25.15 KDa
Source organism: Pseudomonas aeruginosa NCGM2.S1
Expression system: Escherichia coli BL21(DE3)
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

3 bound ligands:
Ligand FE 4 x FE
Ligand ZN 4 x ZN
Ligand FLC 4 x FLC
1 modified residue:
Ligand CSD 4 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P1
Unit cell:
a: 49.99Å b: 75.91Å c: 82.36Å
α: 83.45° β: 75.3° γ: 74.01°
R-values:
R R work R free
0.162 0.162 0.182
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

β-Lactamases and β-Lactamase Inhibitors in the 21st Century.
Tooke et al. (2019)
3 more

1 mention without citation

A Chiral Lanthanide Tag for Stable and Rigid Attachment to Single Cysteine Residues in Proteins for NMR, EPR and Time-Resolved Luminescence Studies.
Herath et al. (2021)