› 4ui2

X-ray diffraction
3.15Å resolution

Crystal structure of the ternary RGMB-BMP2-NEO1 complex

Released:
DOI: 10.2210/pdb4ui2/pdb
PDB entry 4ui2 coloured by chain, front view.
PDB entry 4ui2 coloured by chain, side view.
PDB entry 4ui2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Repulsive guidance molecule is a structural bridge between neogenin and bone morphogenetic protein.
Healey EG, Bishop B, Elegheert J, Bell CH, Padilla-Parra S, Siebold C
Nat Struct Mol Biol 22 458-65 (2015)
PMID: 25938661

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-146287 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Neogenin Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 29.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q92859 (Residues: 852-1102; Coverage: 18%)
Gene names: IGDCC2, NEO1, NGN
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins
Bone morphogenetic protein 2 Chain: B
Molecule details ›
Chain: B
Length: 114 amino acids
Theoretical weight: 12.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12643 (Residues: 283-396; Coverage: 31%)
Gene names: BMP2, BMP2A
Sequence domains: Transforming growth factor beta like domain
Structure domains: Cystine-knot cytokines
Repulsive guidance molecule B Chain: C
Molecule details ›
Chain: C
Length: 122 amino acids
Theoretical weight: 13.32 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6NW40 (Residues: 50-168; Coverage: 30%)
Gene name: RGMB
Sequence domains: Repulsive guidance molecule (RGM) N-terminus
Repulsive guidance molecule B Chain: D
Molecule details ›
Chain: D
Length: 251 amino acids
Theoretical weight: 27.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6NW40 (Residues: 169-410; Coverage: 62%)
Gene name: RGMB
Sequence domains:
Structure domains: Mog1/PsbP, alpha/beta/alpha sandwich

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
2 bound ligands:
Ligand SRT 3 x SRT
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P43212
Unit cell:
a: 120.08Å b: 120.08Å c: 204.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.236
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Targeting iron metabolism in drug discovery and delivery.
Crielaard et al. (2017)
12 more

4 mentions without citation

Structural Biology and Evolution of the TGF-β Family.
Hinck et al. (2016)
3 more