› 4uj3

X-ray diffraction
3Å resolution

Crystal structure of human Rab11-Rabin8-FIP3

Released:
DOI: 10.2210/pdb4uj3/pdb
PDB entry 4uj3 coloured by chain, front view.
PDB entry 4uj3 coloured by chain, side view.
PDB entry 4uj3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of Rab11-FIP3-Rabin8 reveals simultaneous binding of FIP3 and Rabin8 effectors to Rab11.
Vetter M, Stehle R, Basquin C, Lorentzen E
Nat Struct Mol Biol 22 695-702 (2015)
PMID: 26258637

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-130859 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ras-related protein Rab-11A Chains: A, D, G, J, M, P, S, V
Molecule details ›
Chains: A, D, G, J, M, P, S, V
Length: 187 amino acids
Theoretical weight: 21.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62491 (Residues: 4-186; Coverage: 85%)
Gene names: RAB11, RAB11A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Rab-3A-interacting protein Chains: B, E, H, K, N, Q, T, W
Molecule details ›
Chains: B, E, H, K, N, Q, T, W
Length: 195 amino acids
Theoretical weight: 22.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96QF0 (Residues: 286-476; Coverage: 40%)
Gene names: RAB3IP, RABIN8
Rab11 family-interacting protein 3 Chains: C, F, I, L, O, R, U, X
Molecule details ›
Chains: C, F, I, L, O, R, U, X
Length: 66 amino acids
Theoretical weight: 7.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75154 (Residues: 695-756; Coverage: 8%)
Gene names: ARFO1, KIAA0665, RAB11FIP3
Sequence domains: FIP domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

4 bound ligands:
Ligand GNP 8 x GNP
Ligand MG 8 x MG
Ligand SO4 1 x SO4
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 67.498Å b: 165.374Å c: 218.687Å
α: 90° β: 95.93° γ: 90°
R-values:
R R work R free
0.2 0.198 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The intraflagellar transport machinery in ciliary signaling.
Mourão et al. (2016)
4 more

6 mentions without citation

Rab GTPases and their interacting protein partners: Structural insights into Rab functional diversity.
Pylypenko et al. (2018)
5 more