› 4uj4

X-ray diffraction
4.2Å resolution

Crystal structure of human Rab11-Rabin8-FIP3

Released:
DOI: 10.2210/pdb4uj4/pdb
PDB entry 4uj4 coloured by chain, front view.
PDB entry 4uj4 coloured by chain, side view.
PDB entry 4uj4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of Rab11-FIP3-Rabin8 reveals simultaneous binding of FIP3 and Rabin8 effectors to Rab11.
Vetter M, Stehle R, Basquin C, Lorentzen E
Nat Struct Mol Biol 22 695-702 (2015)
PMID: 26258637

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-130861 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ras-related protein Rab-11A Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 185 amino acids
Theoretical weight: 20.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62491 (Residues: 4-186; Coverage: 85%)
Gene names: RAB11, RAB11A
Sequence domains: Ras family
Rab-3A-interacting protein Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 195 amino acids
Theoretical weight: 22.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96QF0 (Residues: 286-476; Coverage: 40%)
Gene names: RAB3IP, RABIN8
Rab11 family-interacting protein 3 Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 66 amino acids
Theoretical weight: 7.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75154 (Residues: 695-756; Coverage: 8%)
Gene names: ARFO1, KIAA0665, RAB11FIP3
Sequence domains: FIP domain

Ligands and Environments

2 bound ligands:
Ligand GNP 4 x GNP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 66.12Å b: 161.701Å c: 192.762Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.252 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Rab GTPases and their interacting protein partners: Structural insights into Rab functional diversity.
Pylypenko et al. (2018)
6 more

1 mention without citation

The substrate specificity of the human TRAPPII complex's Rab-guanine nucleotide exchange factor activity.
Jenkins et al. (2020)