› 4umo

X-ray diffraction
3Å resolution

Crystal Structure of the Kv7.1 proximal C-terminal Domain in Complex with Calmodulin

Released:
DOI: 10.2210/pdb4umo/pdb
PDB entry 4umo coloured by chain, front view.
PDB entry 4umo coloured by chain, side view.
PDB entry 4umo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of a Kv7.1 potassium channel gating module: studies of the intracellular c-terminal domain in complex with calmodulin.
Sachyani D, Dvir M, Strulovich R, Tria G, Tobelaim W, Peretz A, Pongs O, Svergun D, Attali B, Hirsch JA
Structure 22 1582-94 (2014)
PMID: 25441029

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143971 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Potassium voltage-gated channel subfamily KQT member 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 112 amino acids
Theoretical weight: 13.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P51787 (Residues: 352-539; Coverage: 12%)
Gene names: KCNA8, KCNA9, KCNQ1, KVLQT1
Structure domains: Helix Hairpins
Calmodulin-1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

3 bound ligands:
Ligand K 4 x K
Ligand SCN 1 x SCN
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P65
Unit cell:
a: 151.76Å b: 151.76Å c: 56.091Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.213 0.247
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

Modulation of Kv7 channels and excitability in the brain.
Greene et al. (2017)
12 more

3 mentions without citation

Atomistic Insights of Calmodulin Gating of Complete Ion Channels.
Núñez et al. (2020)
2 more