› 4un5

X-ray diffraction
2.4Å resolution

Crystal structure of Cas9 bound to PAM-containing DNA target containing mismatches at positions 1-3

Released:
DOI: 10.2210/pdb4un5/pdb
PDB entry 4un5 coloured by chain, front view.
PDB entry 4un5 coloured by chain, side view.
PDB entry 4un5 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease.
Anders C, Niewoehner O, Duerst A, Jinek M
Nature 513 569-73 (2014)
PMID: 25079318

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118203 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: B
Molecule details ›
Chain: B
Length: 1372 amino acids
Theoretical weight: 158.88 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
TARGET DNA STRAND PROXIMAL FRAGMENT Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.3 KDa
Source organism: synthetic construct
Expression system: Not provided
NON-TARGET DNA STRAND Chain: D
Molecule details ›
Chain: D
Length: 11 nucleotides
Theoretical weight: 3.41 KDa
Source organism: synthetic construct
Expression system: Not provided
TARGET DNA STRAND DISTAL FRAGMENT Chain: E
Molecule details ›
Chain: E
Length: 17 nucleotides
Theoretical weight: 5.22 KDa
Source organism: synthetic construct
Expression system: Not provided
SGRNA Chain: A
Molecule details ›
Chain: A
Length: 83 nucleotides
Theoretical weight: 26.79 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 180.182Å b: 68.072Å c: 190.261Å
α: 90° β: 111.37° γ: 90°
R-values:
R R work R free
0.216 0.215 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

228 review citations

Genome editing. The new frontier of genome engineering with CRISPR-Cas9.
Doudna et al. (2014)
227 more

5 mentions without citation

Epigenome engineering in cancer: fairytale or a realistic path to the clinic?
Falahi et al. (2015)
4 more