› 4urd

Electron Microscopy
7.7Å resolution

Cryo-EM map of Trigger Factor bound to a translating ribosome

Released:
DOI: 10.2210/pdb4urd/pdb
PDB entry 4urd coloured by chain, front view.
PDB entry 4urd coloured by chain, side view.
PDB entry 4urd coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Dynamic Behavior of Trigger Factor on the Ribosome.
Deeng J, Chan KY, van der Sluis EO, Berninghausen O, Han W, Gumbart J, Schulten K, Beatrix B, Beckmann R
J Mol Biol 428 3588-602 (2016)
PMID: 27320387
Related structures: EMD-2695

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141658 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trigger factor Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 12.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A850 (Residues: 1-115; Coverage: 27%)
Gene names: JW0426, b0436, tig
Sequence domains: Bacterial trigger factor protein (TF)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.7Å
Relevant EMDB volumes: EMD-2695
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The chaperone toolbox at the single-molecule level: From clamping to confining.
Avellaneda et al. (2017)
3 more