› 4usx

X-ray diffraction
1.8Å resolution

The Structure of the C-terminal YadA-like domain of BPSL2063 from Burkholderia pseudomallei

Released:
DOI: 10.2210/pdb4usx/pdb
PDB entry 4usx coloured by chain, front view.
PDB entry 4usx coloured by chain, side view.
PDB entry 4usx coloured by chain, top view.
Source organism: Burkholderia pseudomallei K96243
Primary publication:
Selecting soluble/foldable protein domains through single-gene or genomic ORF filtering: structure of the head domain of Burkholderia pseudomallei antigen BPSL2063.
Gourlay LJ, Peano C, Deantonio C, Perletti L, Pietrelli A, Villa R, Matterazzo E, Lassaux P, Santoro C, Puccio S, Sblattero D, Bolognesi M
Acta Crystallogr D Biol Crystallogr 71 2227-35 (2015)
PMID: 26527140

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179086 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hemagglutinin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 371 amino acids
Theoretical weight: 35.84 KDa
Source organism: Burkholderia pseudomallei K96243
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q63TA4 (Residues: 657-992; Coverage: 31%)
Gene name: BPSL2063
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand MG 7 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 63.115Å b: 58.852Å c: 74.407Å
α: 90° β: 104.65° γ: 90°
R-values:
R R work R free
0.17 0.168 0.204
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Current Advances in Burkholderia Vaccines Development.
Wang et al. (2020)
1 more

1 mention without citation

Recent advances in the understanding of trimeric autotransporter adhesins.
Kiessling et al. (2020)