› 4uvk

X-ray diffraction
2.6Å resolution

Cohesin subunit Scc3 from Z. rouxii, 88-1035

Released:
DOI: 10.2210/pdb4uvk/pdb
PDB entry 4uvk coloured by chain, front view.
PDB entry 4uvk coloured by chain, side view.
PDB entry 4uvk coloured by chain, top view.
Source organism: Zygosaccharomyces rouxii
Primary publication:
Structure and function of cohesin's Scc3/SA regulatory subunit.
Roig MB, Löwe J, Chan KL, Beckouët F, Metson J, Nasmyth K
FEBS Lett 588 3692-702 (2014)
PMID: 25171859

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SCD domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 954 amino acids
Theoretical weight: 109.75 KDa
Source organism: Zygosaccharomyces rouxii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5DWM3 (Residues: 88-1035; Coverage: 88%)
Gene name: ZYRO0D15994g
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 73.372Å b: 109.176Å c: 159.029Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Cohesin and its regulation: on the logic of X-shaped chromosomes.
Haarhuis et al. (2014)
12 more

4 mentions without citation

Structural insights into DNA loop extrusion by SMC protein complexes.
Datta et al. (2020)
3 more