› 4uy4

X-ray diffraction
1.86Å resolution

1.86 A structure of human Spindlin-4 protein in complex with histone H3K4me3 peptide

Released:
DOI: 10.2210/pdb4uy4/pdb
PDB entry 4uy4 coloured by chain, front view.
PDB entry 4uy4 coloured by chain, side view.
PDB entry 4uy4 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Talon R, Gileadi C, Johansson C, Burgess-Brown N, Shrestha L, von Delft F, Krojer T, Fairhead M, Bountra C, Arrowsmith CH, Edwards A, Oppermann U

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159412 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Spindlin-4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 24.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q56A73 (Residues: 36-249; Coverage: 86%)
Gene name: SPIN4
Sequence domains: Spin/Ssty Family
Structure domains: Spindlin/Ssty
Histone H3.1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 amino acids
Theoretical weight: 748 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-7; Coverage: 4%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand M3L 2 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 39.072Å b: 51.69Å c: 57.141Å
α: 81.25° β: 89.89° γ: 88°
R-values:
R R work R free
0.203 0.202 0.235
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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