› 4uyj

X-ray diffraction
3.35Å resolution

Crystal structure of a Signal Recognition Particle Alu domain in the elongation arrest conformation

Released:
DOI: 10.2210/pdb4uyj/pdb
PDB entry 4uyj coloured by chain, front view.
PDB entry 4uyj coloured by chain, side view.
PDB entry 4uyj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a signal recognition particle Alu domain in the elongation arrest conformation.
Bousset L, Mary C, Brooks MA, Scherrer A, Strub K, Cusack S
RNA 20 1955-62 (2014)
PMID: 25336584

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153355 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Signal recognition particle 9 kDa protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 85 amino acids
Theoretical weight: 10.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49458 (Residues: 1-85; Coverage: 99%)
Gene name: SRP9
Sequence domains: Signal recognition particle 9 kDa protein (SRP9)
Structure domains: Signal recognition particle alu RNA binding heterodimer, srp9/1
Signal recognition particle 14 kDa protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 107 amino acids
Theoretical weight: 12.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37108 (Residues: 1-107; Coverage: 79%)
Gene name: SRP14
Sequence domains: Signal recognition particle 14kD protein
Structure domains: Signal recognition particle alu RNA binding heterodimer, srp9/1
SRP RNA Chains: R, S
Molecule details ›
Chains: R, S
Length: 110 nucleotides
Theoretical weight: 35.66 KDa

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 16 x MSE
Ligand CCC 2 x CCC

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 103.95Å b: 108.6Å c: 128.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

SRPassing Co-translational Targeting: The Role of the Signal Recognition Particle in Protein Targeting and mRNA Protection.
Kellogg et al. (2021)
1 more

1 mention without citation

Crystal structure of a signal recognition particle Alu domain in the elongation arrest conformation.
Bousset et al. (2014)