› 4v0k

X-ray diffraction
1.44Å resolution

Crystal structure of the CrARL6DN in the GDP bound form

Released:
DOI: 10.2210/pdb4v0k/pdb
PDB entry 4v0k coloured by chain, front view.
PDB entry 4v0k coloured by chain, side view.
PDB entry 4v0k coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Structural basis for membrane targeting of the BBSome by ARL6.
Mourão A, Nager AR, Nachury MV, Lorentzen E
Nat Struct Mol Biol 21 1035-41 (2014)
PMID: 25402481

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108698 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ARF-LIKE SMALL GTPASE Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 18.81 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8JF99 (Residues: 16-180; Coverage: 92%)
Gene name: CHLRE_16g664500v5
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand CD 10 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 65.725Å b: 65.725Å c: 185.377Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.155 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

IFT-Cargo Interactions and Protein Transport in Cilia.
Lechtreck KF. (2015)
16 more

2 mentions without citation

Structural basis for membrane targeting of the BBSome by ARL6.
Mourão et al. (2014)
1 more