› 4w9b

X-ray diffraction
1.28Å resolution

Crystal structure of Gamma-B Crystallin expressed in E. coli based on mRNA variant 1

Released:
DOI: 10.2210/pdb4w9b/pdb
PDB entry 4w9b coloured by chain, front view.
PDB entry 4w9b coloured by chain, side view.
PDB entry 4w9b coloured by chain, top view.
Source organism: Bos taurus
Entry authors: Kudlinzki D, Buhr F, Linhard VL, Jha S, Komar AA, Schwalbe H

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135883 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-crystallin B Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 21.95 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02526 (Residues: 1-175; Coverage: 100%)
Gene name: CRYGB
Sequence domains: Beta/Gamma crystallin
Structure domains: Crystallins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: R32
Unit cell:
a: 123.641Å b: 123.641Å c: 56.548Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.17 0.188
Expression system: Escherichia coli BL21(DE3)

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