› 4wcx

X-ray diffraction
1.59Å resolution

Crystal structure of HydG: A maturase of the [FeFe]-hydrogenase

Released:
DOI: 10.2210/pdb4wcx/pdb
PDB entry 4wcx coloured by chain, front view.
PDB entry 4wcx coloured by chain, side view.
PDB entry 4wcx coloured by chain, top view.
Source organism: Thermoanaerobacter italicus Ab9
Primary publication:
X-ray crystallographic and EPR spectroscopic analysis of HydG, a maturase in [FeFe]-hydrogenase H-cluster assembly.
Dinis P, Suess DL, Fox SJ, Harmer JE, Driesener RC, De La Paz L, Swartz JR, Essex JW, Britt RD, Roach PL
Proc Natl Acad Sci U S A 112 1362-7 (2015)
PMID: 25605932

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112545 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Radical SAM core domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 480 amino acids
Theoretical weight: 55.22 KDa
Source organism: Thermoanaerobacter italicus Ab9
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D3T7F1 (Residues: 1-466; Coverage: 100%)
Gene name: Thit_0582
Sequence domains:

Ligands and Environments

7 bound ligands:
Ligand SF4 4 x SF4
Ligand MET 1 x MET
Ligand FE 1 x FE
Ligand ALA 1 x ALA
Ligand H2S 2 x H2S
Ligand NA 3 x NA
Ligand SAM 1 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 54.13Å b: 56.19Å c: 84.92Å
α: 89.59° β: 83.62° γ: 66.84°
R-values:
R R work R free
0.18 0.18 0.213
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Synthesis of Diiron(I) Dithiolato Carbonyl Complexes.
Li et al. (2016)
11 more

16 mentions without citation

Biosynthesis of the catalytic H-cluster of [FeFe] hydrogenase: the roles of the Fe-S maturase proteins HydE, HydF, and HydG.
Britt et al. (2020)
15 more