› 4wfe

X-ray diffraction
2.5Å resolution

Human TRAAK K+ channel in a K+ bound conductive conformation

Released:
DOI: 10.2210/pdb4wfe/pdb
PDB entry 4wfe coloured by chain, front view.
PDB entry 4wfe coloured by chain, side view.
PDB entry 4wfe coloured by chain, top view.
Source organisms:
Primary publication:
Physical mechanism for gating and mechanosensitivity of the human TRAAK K+ channel.
Brohawn SG, Campbell EB, MacKinnon R
Nature 516 126-30 (2014)
PMID: 25471887

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230399 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Potassium channel subfamily K member 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 32.57 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9NYG8 (Residues: 1-264; Coverage: 67%)
Gene names: KCNK4, TRAAK
Sequence domains: Ion channel
Structure domains: Helix Hairpins
ANTI-TRAAK ANTIBODY 13E9 FAB FRAGMENT LIGHT CHAIN Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.04 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
ANTI-TRAAK ANTIBODY 13E9 FAB FRAGMENT HEAVY CHAIN Chains: E, G
Molecule details ›
Chains: E, G
Length: 217 amino acids
Theoretical weight: 23.47 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand K 6 x K
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 80.795Å b: 138.522Å c: 96.843Å
α: 90° β: 95.08° γ: 90°
R-values:
R R work R free
0.201 0.199 0.234
Expression systems:
  • Komagataella pastoris
  • Mus musculus

Quick links

Citations

44 review citations

Mechanically Activated Ion Channels.
Ranade et al. (2015)
43 more

14 mentions without citation

Structure of potassium channels.
Kuang et al. (2015)
13 more