› 4wfg

X-ray diffraction
3Å resolution

Human TRAAK K+ channel in a Tl+ bound conductive conformation

Released:
DOI: 10.2210/pdb4wfg/pdb
PDB entry 4wfg coloured by chain, front view.
PDB entry 4wfg coloured by chain, side view.
PDB entry 4wfg coloured by chain, top view.
Source organisms:
Primary publication:
Physical mechanism for gating and mechanosensitivity of the human TRAAK K+ channel.
Brohawn SG, Campbell EB, MacKinnon R
Nature 516 126-30 (2014)
PMID: 25471887

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230401 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Potassium channel subfamily K member 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 32.57 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9NYG8 (Residues: 1-264; Coverage: 67%)
Gene names: KCNK4, TRAAK
Sequence domains: Ion channel
Structure domains: Helix Hairpins
ANTI-TRAAK ANTIBODY 13E9 FAB FRAGMENT LIGHT CHAIN Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.04 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
ANTI-TRAAK ANTIBODY 13E9 FAB FRAGMENT HEAVY CHAIN Chains: E, G
Molecule details ›
Chains: E, G
Length: 217 amino acids
Theoretical weight: 23.47 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand TL 8 x TL
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 80.798Å b: 138.76Å c: 96.317Å
α: 90° β: 94.52° γ: 90°
R-values:
R R work R free
0.204 0.202 0.236
Expression systems:
  • Komagataella pastoris
  • Mus musculus

Quick links

Citations

45 review citations

Mechanically Activated Ion Channels.
Ranade et al. (2015)
44 more

4 mentions without citation

Structural Insights into the Mechanisms and Pharmacology of K2P Potassium Channels.
Natale et al. (2021)
3 more