› 4wk1

X-ray diffraction
1.98Å resolution

Crystal structure of Staphylococcus aureus PstA in complex with c-di-AMP

Released:
DOI: 10.2210/pdb4wk1/pdb
PDB entry 4wk1 coloured by chain, front view.
PDB entry 4wk1 coloured by chain, side view.
PDB entry 4wk1 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus COL
Primary publication:
c-di-AMP recognition by Staphylococcus aureus PstA.
Müller M, Hopfner KP, Witte G
FEBS Lett 589 45-51 (2015)
PMID: 25435171

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101619 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein from nitrogen regulatory protein P-II (GLNB) family Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2WXZ7 (Residues: 1-109; Coverage: 100%)
Gene name: SACOL0525
Sequence domains: Cyclic-di-AMP receptor
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand 2BA 1 x 2BA
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R32
Unit cell:
a: 68.1Å b: 68.1Å c: 135.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.188 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

A jack of all trades: the multiple roles of the unique essential second messenger cyclic di-AMP.
Commichau et al. (2015)
10 more

3 mentions without citation

Cyclic di-AMP, a second messenger of primary importance: tertiary structures and binding mechanisms.
He et al. (2020)
2 more