› 4wum

X-ray diffraction
1.77Å resolution

X-ray crystal structure of Chalcone Synthase from Freesia hybrida

Released:
DOI: 10.2210/pdb4wum/pdb
PDB entry 4wum coloured by chain, front view.
PDB entry 4wum coloured by chain, side view.
PDB entry 4wum coloured by chain, top view.
Source organism: Freesia hybrid cultivar
Primary publication:
Molecular and Biochemical Analysis of Chalcone Synthase from Freesia hybrid in flavonoid biosynthetic pathway.
Sun W, Meng X, Liang L, Jiang W, Huang Y, He J, Hu H, Almqvist J, Gao X, Wang L
PLoS One 10 e0119054 (2015)
PMID: 25742495

Function and Biology Details

Reaction catalysed: 
3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124330 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 389 amino acids
Theoretical weight: 42.64 KDa
Source organism: Freesia hybrid cultivar
Expression system: Escherichia coli
UniProt:
  • Canonical: G3FJ87 (Residues: 1-389; Coverage: 100%)
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 79.43Å b: 79.51Å c: 232.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.227
Expression system: Escherichia coli

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Citations

3 review citations

Recent advances on the development and regulation of flower color in ornamental plants.
Zhao et al. (2015)
2 more

4 mentions without citation

Molecular and Biochemical Analysis of Chalcone Synthase from Freesia hybrid in flavonoid biosynthetic pathway.
Sun et al. (2015)
3 more