› 4x1u

X-ray diffraction
1.87Å resolution

The structure of AhpE from Mycobacterium tuberculosis revisited

Released:
DOI: 10.2210/pdb4x1u/pdb
PDB entry 4x1u coloured by chain, front view.
PDB entry 4x1u coloured by chain, side view.
PDB entry 4x1u coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Revisiting sulfur H-bonds in proteins: The example of peroxiredoxin AhpE.
van Bergen LA, Alonso M, Palló A, Nilsson L, De Proft F, Messens J
Sci Rep 6 30369 (2016)
PMID: 27468924

Function and Biology Details

Reaction catalysed: 
Mycoredoxin + ROOH = mycoredoxin disulfide + H(2)O + ROH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkyl hydroperoxide reductase E Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 17.54 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WIE3 (Residues: 1-152; Coverage: 99%)
Gene names: MTCY427.19c, Rv2238c, ahpE
Sequence domains: AhpC/TSA family
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand GOL 5 x GOL
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: I4
Unit cell:
a: 147.994Å b: 147.994Å c: 33.711Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.183
Expression system: Escherichia coli

Quick links

Citations

2 review citations

C-H⋯S hydrogen bonding interactions.
Fargher et al. (2022)
1 more

1 mention without citation

Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities.
Bolduc et al. (2021)